A sustainable and versatile in vitro model for human skin diseases

Samenvatting

To study human epidermal development and diseases, a model that can faithfully recapitulate embryonic epidermal development is required. For this purpose, my group successfully established an in vitro model using human induced pluripotent stem cells (iPSCs). In this model, human iPSCs are differentiated towards epidermal keratinocytes (iKCs). The iKCs display classical normal epidermal KC characteristics. However our molecular analyses also showed that these iKCs are immature. To improve this in vitro system, we will combine the expertise of the two groups (the Zhou lab in Nijmegen, NL and the Hennies lab in University of Huddersfield, UK) in dermatological research, particularly in iPSCs differentiation into epidermal keratinocytes, 3D skin models and transcriptomics and epigenomics analyses. The main goal of this visitor travel project is to improve strategies to achieve efficient differentiation of iPSCs and to obtain iKCs that resemble KCs derived from the epidermis. We will assess the improved in vitro differentiation protocol by quantifying its efficiency to derive iKCs that resemble epidermal KCs using two standards: 1) (single-cell) RNA-seq for their molecular signatures (expertise in the Zhou lab); 2) their ability to generate 3-dimensional organotypic equivalents, a gold standard of high-quality KCs (expertise in the Hennies lab). This work will be carried out by a postdoctoral fellow Dulce Lima Cunha in the Hennies lab.

Kenmerken

Projectnummer

040.11.699

Hoofdaanvrager

Dr. J.H. Zhou PhD

Verbonden aan

Radboud Universiteit Nijmegen, Faculteit der Natuurwetenschappen, Wiskunde en Informatica, Radboud University Nijmegen Medical Center

Looptijd

16/08/2019 tot 30/09/2019