Disease biomarker detection for Ebola and Lassa virus induced vasculopathy

Samenvatting

The Ebola and Lassa recent epidemic in West Africa was a major health crisis of unprecedented magnitude and impact, causing thousands of deaths and destabilizing several countries. Ebola and Lassa are two virulent diseases with high mortality rates (50% and 20% respectively). Death occurs due to vascular integrity impairment caused by the viruses that leads to severe blood loss (known as hemorrhagic shock syndrome). How the viruses cause this process is not yet fully understood, therefore, no specific treatment exists to this date. This is due to the lack of in-vitro models, as well as sensitive detection tools. In this project, we aim to uncover the metabolic alterations to endothelial cells caused by these viruses, and by extension, aid in the efforts of formulating a more effective treatment. To achieve this, the organ-on-a-chip technology will be used to model the diseases, then, the metabolic alterations will be studied using sensitive mass spectrometry approaches and will be correlated with the extent of vascular integrity loss. The resulting novel biomarkers that will be discovered will give us a better understanding of the virus effect for early detection, as well as aid in developing a more effective and specific treatment.

Kenmerken

Projectnummer

NWA.1228.191.329

Hoofdaanvrager

Y. Abouleila

Verbonden aan

Universiteit Leiden, Faculteit der Wiskunde en Natuurwetenschappen, Leiden Academic Centre for Drug Research

Looptijd

01/10/2019 tot 30/09/2020