SafeChassis: Implementing and Assessing Safeguards for Lifestyle Engineering of a Versatile Industrial Chassis


This project will develop technical safeguards to limit environmental, health and safety effects of pseudomonas putida, a microbe that is now used as a chassis in a number of industrial processes. This microbe is currently being redesigned to further enhance its commercial value by, among other, uncoupling growth from production and by transforming it into a facultative anaerobe. The safeguards under consideration would be based on prior work on multiple nutrient dependencies on synthetic amino acids and on recoding strategies to limit lateral gene flow.
This project will develop credible testing methods to evaluate the effectiveness of technical safeguards to limit environmental effects with early engagement of civil society, regulators and environmental scientists. The project is unique in engaging with a broader-than-typical array of stakeholders at the front end of the project, with workshops to inform the design of testing methods and to assist in the development of risk assessment methods.


Project number


Main applicant

Prof. dr. ing. V.A.P. Martins dos Santos

Affiliated with

Wageningen University & Research, Plantenwetenschappen, Plant Developmental Biology

Team members

E. Asín Garcia, Dr. A. Kallergi, Dr. J.G. Zwier


01/12/2017 to 31/08/2019