Super-resolution endo-microscopy


Light microscopy has been a key tool for biological and medical research for centuries, but the limited penetration due to light scattering has restricted its in vivo imaging ability to near-surface region. To overcome this constraint endoscopic techniques based on miniature optical probes have been developed. However, even nowadays high-resolution (<500 nm) multifunctional imaging deep into organs or biotissue (below 1 mm) remains an elusive goal.
In this VENI program, I will create the next generation of optical endoscopic methods fully applicable for biovisualization: super-resolution fluorescent imaging and high-resolution chemically-selective label-free imaging. I will develop the breakthrough technologies by integrating wavefront shaping of light in unique multimode photonic crystal fiber probes with advanced optical microscopy methods, such as Raman and super-resolution microscopy. Implementation of cutting-edge methods of optical microscopy in a multimode fiber format is very challenging but extremely beneficial. It will allow drastical improvement of the functionality of fiber endoscopes and provide resolution below the diffraction limit at any depth accessible by endoscopy inside living tissue.
The results of this work potentially have broad applications in life sciences and medicine, opening a new era of bioimaging in endoscopic format. New endomicroscopy methods will bring optical biopsy to the next level, by providing minimally-invasive microscopic images of tissues at unparalleled resolution and without artificial biomarkers. It will allow visualization of tiny cell features deep inside living organisms or performing longitudinal observation of dynamic phenomena in their natural environments. The new methods can be used for mass production of a new generation of fiber-optic microscopes. Being easy-to-use, affordable and compact, the new system can be deployed at any Lab and Medical center.


Project number


Main applicant

L.V. Amitonova

Affiliated with

Universiteit Twente, Faculteit der Technische Natuurwetenschappen (TNW), Applied Physics


01/12/2017 to 31/08/2020