Microbial Diversity in the Deepest Hypersaline Anoxic Lakes

Summary

The deep-sea hypersaline anoxic lakes represent a special type of environments different from any known ecosystems and one of the last frontiers of the undiscovered biosphere that challenges the life science today. A number of very initial attempts to characterise the indigenous microbial consortia, gained valuable and promising background information on the presence of novel and unique pro- and eukaryotic organisms in these extreme environments. Up to date, none of them have ever been isolated and obtained in pure culture, thus, their metabolic features and their role within their natural ecosystems are still unknown. MIDDLE Project will concentrate for the first time on the comprehensive molecular biodiversity study of two DHAL environments, namely Discovery (pure bischofite) and L’Atalante - Urania (pure halite) as typical sites for different extreme environments (athalassic and thalassic, respectively). The main objectives and milestones of the proposed study are
(i) exploration of the biodiversity of pro- and eukaryotic microorganisms thriving in deep Mediterranean hypersaline anoxic lakes on both phylogenetic and functional gene analysis levels.
(ii) understanding the biogeochemical pathways that sustain DHALs microbial metabolic network systems and the possible interactions of prokaryotes with protists
(iii) exploration and exploitation of the genomic richness hidden within DHALs
Further development of molecular techniques like metagenome and functional genomic is foreseen in the frame of MIDDLE to get inside of astonishing DHAL genomic pool and to mine for novel biologically active products of industrial and technological importance. The application of biogeochemical, analytical, biochemical, microbiological, molecular biological studies for detailed bioinformatic characterization of DHALs microbial metabolic network is foreseen in the frame of the MIDDLE Proposal.

Output

Scientific article

  • G.J. de Lange, et al(2005): The Enigma of prokaryotic life in deep hypersaline anoxic basins. Science pp. 121 - 123
  • G.J. de Lange, et al(2006): Stratified prokaryote network in the oxic-anoxic transition of a deep-sea halocline. Nature
  • A. Robertson, et all(2007): Global Look at Salt Giants Eos 88 pp. 177 - 179
  • G.J. de Lange, et al(2008): scanning as an estimate of the marine organic carbon content of sediment cores. Geochem. Geophys. Geosyst., 9, Q05009, doi:10.1029/2007GC001932 . Geochem. Geophys. Geosyst., 9, Q05009, doi:10.1029/2007GC001932 pp. Q05009, doi:10.1029/2007GC001932
  • G.J. de Lange, et al(2008): A preliminary assessment of the composition of evaporites underlying the eastern mediterranean seafloor using pore water. In: CIESM Monographs 33 (2008): 111-114. CIESM Monographs pp. 111 - 114
  • G.J. de Lange, et al(2008): Synchronous basin-wide formation and redox-controlled preservation of a Mediterranean sapropel. Nature Geo 1, 606-610. Nature Geo 1, pp. 606 - 610
  • L.J. Lourens, et all(2008): Fluid displacive resin embedding of laminated sediments: preserving trace metals for high-resolution paleoclimate investigations Limnol. Oceanogr Methods pp. 16 - 22
  • G.J. de Lange, et al(2008): Past and present variations in the C-cycle and related changes in eastern Mediterranean C deposition and preservation. In: CIESM Monographs 36: Impacts of acidification on biological, chemical and physical systems in the Mediterranean and Black Seas Mento CIESM Monographs 36 pp. 39 - 42
  • C.L. Casalino, G. de Lange(2009): Deep eastern Mediterranean hypersaline anoxic brine lakes sampled during 2008 DOPPIO cruise with RV Pelagia Geophysical Resaerch Abstracts ISSN: EGU2009-6771-1.
  • C.L. Casalino, G.J. de Lange(2009): Geochemical charcterisation of hypersaline anoxic brine basins from aestern mediterrranean Sea Geochimica Cosmo Acta pp. A197 - A197
  • G.J. de Lange, et al(2009): Sulfur cycling and methanogenesis primarily drive microbial colonization of the highly sulfidic Urania deep hypersaline basin. doi: 10.1073/pnas.0811984106; PNAS June 9, 2009 vol. 106 no. 23 9151-9156. doi: 10.1073/pnas.0811984106; PNAS June 9, 2009 vol. 106 no. pp. 23 9151 - 9156.
  • W. Krijgsman, et al(2010): Messinian Salinity Crisis: A novel unifying shallow gypsum / deep dolomite formation mechanism. Mar.Geol. pp. 273 - 277
  • G.J. de Lange, et al(2013): Anammox bacterial populations in deep marine hypersaline gradient systems. Extremophiles pp. 289 - 299

Publications for the general public

  • G.J. de Lange, et al(2006): Holocene laminations in Mediterranean brine sediments - unlocking a new high resolution climate archive
  • G.J. de Lange, et al(2006): Geochemical composition and origin for fluid and gas fluxes at eastern Mediterranean mud volcanoes.
  • G.J. de Lange, et al(2006): Composition/origin of fluids and gas and diagenetic observed and potential features for pre/post & Messinian deposits'.
  • W. Krijgsman, et al(2009): Gypsum precipitation in the Mediterranean during the first stage of the Messinian salinity crisis.
  • W. Krijgsman, et al(2009): Gypsum precipitation in the Mediterranean during the first stage of the Messinian salinity crisis.
  • G.J. de Lange, et al(2009): Sea floor mapping and correlated sismostratigraphic evidences on some selected Mediterranean Basin Sites.
  • G.J. de Lange, et al(2009): Sea floor mapping and correlated sismostratigraphic evidences on some selected Mediterranean Basin Sites
  • G.J. de Lange, et al(2010): Mediterranean deep anoxic hypersaline basins: an overview.
  • G.J. de Lange, et al(2010): Composition and origin of hypersaline eastern Mediteranean brines.
  • G.J. de Lange(2010): Composition and origin of hypersaline eastern Mediteranean brines Hermione/brines.
  • G.J. de Lange(2010): Euro-Marc versus Euro-Deep: Geo- versus Bio-diversity !at.
  • G.J. de Lange, et al(2010): Composition And Origin Of Hypersaline Eastern Mediterranean Brines With Emphasis To Urania Basin.
  • W. Krijgsman, et al(2010): A unifying biogeochemical mechanism for shallow gypsum vs. Deep dolomite formation during the messinian salinity crisis.
  • G.J. de Lange, et al(2011): Habitat Mapping in extreme Deep Sea Environment: geosphere and biosphere interaction in deep anoxic basins.
  • W. Krijgsman, et al(2011): A novel unifying mechanism for shallow gypsum versus deep dolomite formation during the first phase of the Messinian Salinity Crisis.
  • G.J. de Lange, et al(2012): Geochemical overview of the deep anoxic hypersaline basins from east Mediterranean Sea.
  • W. Krijgsman, et al(2013): Primary Messinian Salinity Crisis shallow gypsum vs. deep dolomite formation: A unifying biogeochemical mechanism.
  • G.J. de Lange(2014): Introduction to Biogeochemistry and paleoceanography of Sapropels and Messinian Salinity Crisis evaporates
  • G.J. de Lange, et al(2014): Methanotrophy at the interface between Mediterranean seawater and the MgCl2 dominated Kryos brine lake - proof of life in an extreme environment.
  • W. Krijgsman, et al(2014): Primary Evaporites for the Messinian Salinity Crisis: the shallow gypsum vs. deep dolomite formation paradox solved.
  • G.J. de Lange(2014): Introduction to Biogeochemistry and paleoceanography of Sapropels and Messinian Salinity Crisis evaporites.

Details

Project number

855.01.132

Main applicant

Prof. dr. G.J. de Lange

Affiliated with

Universiteit Utrecht, Faculteit Geowetenschappen, Departement Aardwetenschappen

Team members

Mw. C.L. Casalino, Mw. C.M. Laurent MSc

Duration

01/09/2008 to 17/10/2013