Detailed project information

Title	:	De novo genome assembly of the flatworm Macrostomum lignano
Applicant	:	Dr. E. Berezikov
Research institute	:	Hubrecht Instituut voor Ontwikkelingsbiologie en Stamcelonderzoek
Duration	:	02/12/2010 tot 06/06/2011
Strategic goal	:	Infrastructuur
Subsidy	:	Computing Time National Computing Facilities

Summary

I?m currently leading an effort to sequence

the genome of flatworm Macrstomum lignano, and this project is financed by Hubrecht Institute. The estimated genome size of M. lignano is 600 Mb, and we are utilizing second-generation sequencing technologies (454, Illumina/Solexa, SOLiD) to produce ~60x coverage of the genome in order to generate a high quality hybrid genome assembly.

We have recently generated 40x genome coverage sequencing data for M. lignano genome, and assembling this amount of data using existing genome assembly software requires a system with large amount of RAM (~200GB). The computational cluster at Hubrecht Institute is limited to nodes with 64GB RAM. I therefore apply for access to the Huygens system as a PILOT project.